Phylogenetics and dating with irish dating georgia


02-Dec-2017 00:18

The more than 500 species in nine genera are distributed mainly in South America, Australasia, and Southeast Asia with outliers in Madagascar.The family is absent from continental Africa, Europe, and North America.Inferred parsimony-informative indels were scored as unordered two-state characters.

Eocene leaf fossils of Elaeocarpaceae are abundant in southern Australia, suggesting the family was widespread and diverse in the early Tertiary on that continent (Christophel and Greenwood, 1987 are endemic to South America.Each 25-μL reaction contained 200 μM of each primer, 200 μM of each d NTP, 2.5 μL 10× buffer (Bioline, Luckenwalde, Germany), 1.5–3.0 mmol Mg Cl region, and reaction mixtures were incubated at 94°C for 3 min, then subjected to 25 cycles of the following profile: denaturation for 30 s at 94°C, annealing for 30 s at 52 or 55°C, and extension for 30 s at 72°C. Products from one individual from each of the two known populations of this species were cloned by using the p GEM–T Easy Vector System (Promega, Madison, Wisconsin, USA) in accordance with the manufacturer's protocol.



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